[Fuuk-ext-l] Invitation to Seminar of Institute of Physics, MFF UK, on Tue 7.12. from 14:00, speaker RNDr. Hana Lísalová, Ph.D.

[Jaroslav Hamrle]

Dear colleagues,

It is my pleasure to invite you to Seminar of Institute of Physics of 
Charles University, entitled:

*Advanced functional biointerface for ultra-sensitive detection of 
coronavirus in crude clinical samples*

Speaker: RNDr. Hana Lísalová, Ph.D. 
<https://www.fzu.cz/lide/rndr-hana-lisalova-phd>

Time:  Tuesday 7.12.2021 from 14:00

Place: Seminar room of Institute of Physics F285 (atelier) , second 
floor, Ke Karlovu 5, Prague

/Abstract:
/

/New analytical techniques that overcome major drawbacks of current 
routinely used viral infection diagnosis methods, i.e., the long 
analysis time and laboriousness of qRT-PCR and the insufficient 
sensitivity of “antigen tests”, are urgently needed in the context of 
SARS-CoV-2 and other highly contagious viruses. Here, we report on an 
antifouling terpolymer-brush biointerface that enables the rapid and 
sensitive detection of SARS-CoV-2 in untreated clinical samples. The 
developed iointerface carries a tailored composition of zwitterionic and 
non-ionic moieties. and allows for the significant improvement of 
antifouling capabilities when postmodified with biorecognition elements 
and exposed to in complex media. When deployed on a surface of 
piezoelectric sensor and postmodified with human-cell-expressed 
antibodies specific to nucleocapsid (N) protein of SARS-CoV-2, it made 
possible the quantitative analysis of untreated samples by a direct 
detection assay format without the need of additional amplification 
steps. Natively occurring N-protein-vRNA complexes, usually disrupted 
during the sample pre-treatment steps, were detected in the untreated 
clinical samples. This biosensor design improved the bioassay 
sensitivity to a clinically relevant limit of detection (LOD) of 
1.3×10^4 PFU/mL within a detection time of only 20 minutes. The high 
specificity towards N-protein-vRNA complexes was validated both by mass 
spectrometry and qRT-PCR. The performance characteristics were confirmed 
by qRT-PCR through a comparative study using a set of clinical 
nasopharyngeal swab samples.  We further demonstrate the extraordinary 
performance of developed approach in a large-scale biosensor and qRT-PCR 
comparative study on a the set of 550 surface swab samples collected 
from the means of public transportation. The results highlight the 
potential of developed method to serve as a generic platform a wide 
range of biosensing applications./

With my best regards

Jaroslav Hamrle


-- 
------------------------------------------------------------------
Mgr. Jaroslav Hamrle, Ph.D.
Institute of Physics, room F232
Faculty of Mathematics and Physics
Charles University
Ke Karlovu 5
121 16 Prague
Czech Republic

tel: +420-95155 1340
email: hamrle na karlov.mff.cuni.cz
------------------------------------------------------------------

------------- další část ---------------
HTML příloha byla odstraněna...
URL: <http://lists.karlov.mff.cuni.cz/pipermail/fuuk-ext-l/attachments/20211206/31d2063b/attachment-0001.htm>
------------- další část ---------------
A non-text attachment was scrubbed...
Name: MFF-abstract-Lisalova-1.docx
Type: application/vnd.openxmlformats-officedocument.wordprocessingml.document
Size: 266189 bytes
Desc: [žádný popis není k dispozici]
URL: <http://lists.karlov.mff.cuni.cz/pipermail/fuuk-ext-l/attachments/20211206/31d2063b/attachment-0001.bin>