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<p>Dear colleagues,</p>
<p>It is my pleasure to invite you to Seminar of Institute of
Physics of Charles University, entitled:</p>
<b>Advanced functional biointerface for ultra-sensitive detection of
coronavirus in crude clinical samples</b><br>
<p>Speaker: <a moz-do-not-send="true"
href="https://www.fzu.cz/lide/rndr-hana-lisalova-phd">RNDr. Hana
Lísalová, Ph.D.</a> <br>
<br>
Time: Tuesday 7.12.2021 from 14:00<br>
<br>
Place: Seminar room of Institute of Physics F285 (atelier) ,
second floor, Ke Karlovu 5, Prague</p>
<p><i>Abstract:<br>
</i></p>
<i>New analytical techniques that overcome major drawbacks of
current routinely used viral infection diagnosis methods, i.e.,
the long analysis time and laboriousness of qRT-PCR and the
insufficient sensitivity of “antigen tests”, are urgently needed
in the context of SARS-CoV-2 and other highly contagious viruses.
Here, we report on an antifouling terpolymer-brush biointerface
that enables the rapid and sensitive detection of SARS-CoV-2 in
untreated clinical samples. The developed iointerface carries a
tailored composition of zwitterionic and non-ionic moieties. and
allows for the significant improvement of antifouling capabilities
when postmodified with biorecognition elements and exposed to in
complex media. When deployed on a surface of piezoelectric sensor
and postmodified with human-cell-expressed antibodies specific to
nucleocapsid (N) protein of SARS-CoV-2, it made possible the
quantitative analysis of untreated samples by a direct detection
assay format without the need of additional amplification steps.
Natively occurring N-protein-vRNA complexes, usually disrupted
during the sample pre-treatment steps, were detected in the
untreated clinical samples. This biosensor design improved the
bioassay sensitivity to a clinically relevant limit of detection
(LOD) of 1.3×10^4 PFU/mL within a detection time of only 20
minutes. The high specificity towards N-protein-vRNA complexes was
validated both by mass spectrometry and qRT-PCR. The performance
characteristics were confirmed by qRT-PCR through a comparative
study using a set of clinical nasopharyngeal swab samples. We
further demonstrate the extraordinary performance of developed
approach in a large-scale biosensor and qRT-PCR comparative study
on a the set of 550 surface swab samples collected from the means
of public transportation. The results highlight the potential of
developed method to serve as a generic platform a wide range of
biosensing applications.</i><br>
<p>With my best regards</p>
<p> Jaroslav Hamrle <br>
</p>
<p><br>
</p>
<pre class="moz-signature" cols="72">--
------------------------------------------------------------------
Mgr. Jaroslav Hamrle, Ph.D.
Institute of Physics, room F232
Faculty of Mathematics and Physics
Charles University
Ke Karlovu 5
121 16 Prague
Czech Republic
tel: +420-95155 1340
email: <a class="moz-txt-link-abbreviated" href="mailto:hamrle@karlov.mff.cuni.cz">hamrle@karlov.mff.cuni.cz</a>
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