[Fuuk-ext-l] Seminar TODAY at 14:00, speaker RNDr. Pavel Malý, Ph.D.

[Jaroslav Hamrle]

Dear colleagues,

It is my pleasure to recall my invitation to ours today's Seminar of 
Institute of Physics of Charles University, entitled:

*Fluorescence-detected transient absorption microscopy*

/Abstract:/

/Fluorescence microscopy is a widely popular imaging technique with many 
variants. While the fluorescence detection leads to high sensitivity, it 
also comes with its limitations. Due to the nature of the signal, the 
time resolution is limited by the relatively slow rate of spontaneous 
emission of the excited sample. Such limitation does not apply to 
ultrafast nonlinear coherent spectroscopy techniques such as transient 
absorption, which can track the photo-induced excitation dynamics on 
femtosecond timescale. However, these techniques typically require 
volume samples and are detected coherently./

///In this talk, I will introduce a combination of the two approaches: 
coherent transient absorption (pump--probe) spectroscopy detected by 
fluorescence.[1] This technique retains the time resolution given by the 
excitation pulses, while enjoying the sensitivity of fluorescence 
detection. We directly compared the detection using the two observables 
on diverse samples such as molecular dimers, core-shell quantum dots and 
fluorescent proteins, showing a degree of complementarity between the 
approaches. While useful already in a volume sample, the 
fluorescence-detected transient absorption finds most applications in a 
microscope. I will show our first measurements of transient spectral 
dynamics of single TDI molecules, and outline the motivation and future 
plans for fluorescence-detected transient absorption microscopy imaging 
experiments./
///
//[1] P. Malý and T. Brixner, Fluorescence-detected pump--probe 
spectroscopy, Angewandte Chemie Int. Ed., 60, 18867 (2021)/

Speaker: RNDr. Pavel Malý, Ph.D. <http://biophysics.mff.cuni.cz/Pavel_Maly/>

Time: *Tuesday 12.10. from 14:00*

Place: Seminar room of Institute of Physics F285 (atelier) , second 
floor, Ke Karlovu 5, Prague

With my best regards

Jaroslav Hamrle

-- 
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Mgr. Jaroslav Hamrle, Ph.D.
Institute of Physics, room F232
Faculty of Mathematics and Physics
Charles University
Ke Karlovu 5
121 16 Prague
Czech Republic

tel: +420-95155 1340
email: hamrle na karlov.mff.cuni.cz
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