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<p>Dear colleagues,</p>
<p>It is my pleasure to recall my invitation to ours today's Seminar
of Institute of Physics of Charles University, entitled:</p>
<p><b>Fluorescence-detected transient absorption microscopy</b></p>
<p><i>Abstract:</i></p>
<p align="justify"><i>Fluorescence microscopy is a widely popular
imaging technique with many variants. While the fluorescence
detection leads to high sensitivity, it also comes with its
limitations. Due to the nature of the signal, the time
resolution is limited by the relatively slow rate of spontaneous
emission of the excited sample. Such limitation does not apply
to ultrafast nonlinear coherent spectroscopy techniques such as
transient absorption, which can track the photo-induced
excitation dynamics on femtosecond timescale. However, these
techniques typically require volume samples and are detected
coherently.</i></p>
<p><i> </i><i>In this talk, I will introduce a combination of the
two approaches: coherent transient absorption (pump--probe)
spectroscopy detected by fluorescence.[1] This technique retains
the time resolution given by the excitation pulses, while
enjoying the sensitivity of fluorescence detection. We directly
compared the detection using the two observables on diverse
samples such as molecular dimers, core-shell quantum dots and
fluorescent proteins, showing a degree of complementarity
between the approaches. While useful already in a volume sample,
the fluorescence-detected transient absorption finds most
applications in a microscope. I will show our first measurements
of transient spectral dynamics of single TDI molecules, and
outline the motivation and future plans for
fluorescence-detected transient absorption microscopy imaging
experiments.</i><br>
<i> </i><i><br>
</i><i>[1] P. Malý and T. Brixner, Fluorescence-detected
pump--probe spectroscopy, Angewandte Chemie Int. Ed., 60, 18867
(2021)</i></p>
<p>Speaker: <a href="http://biophysics.mff.cuni.cz/Pavel_Maly/">RNDr.
Pavel Malý, Ph.D.</a><br>
</p>
<p>Time: <b>Tuesday 12.10. from 14:00</b> <br>
</p>
<p>Place: Seminar room of Institute of Physics F285 (atelier) ,
second floor, Ke Karlovu 5, Prague<br>
</p>
<p>With my best regards</p>
Jaroslav Hamrle
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Mgr. Jaroslav Hamrle, Ph.D.
Institute of Physics, room F232
Faculty of Mathematics and Physics
Charles University
Ke Karlovu 5
121 16 Prague
Czech Republic
tel: +420-95155 1340
email: <a class="moz-txt-link-abbreviated" href="mailto:hamrle@karlov.mff.cuni.cz">hamrle@karlov.mff.cuni.cz</a>
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